Process for expressing a isomaltulose synthetase using the gene originated from Erwinia rhapontici

摘要

PURPOSE: Provided is an expression method of isomaltulose synthease massively by cloning isomaltulose synthease gene derived from Erwinia rhapontici, then introducing it into E. coli. CONSTITUTION: The isomaltulose synthease gene is obtained from Erwinia rhapontici by southern hybridization and colony hybridization. The whole nucleotide sequence of its DNA is 1803bp and represented by SEQ ID NO:1. The nucleotide sequence is cloned into an expression vector pET16b and E.coli is transformed with the vector. The transformant, EcoliETpal(KCCM-1206), is cultured in an LB medium to produce isomaltulose synthease, where the expression of the isomaltulose synthease in E.coli transformant is induced by IPTG(Isopropyl-beta-D-thiogalactoside); and the LB medium is an agar plate medium containing LB agar, 3-5% of sugar, 0.3-0.5% of beef extract, 0.9-1.1% of bacto peptone, and 1.3-1.7% of agar composition.