DETECTION KIT OF THYROID HORMONE USING RIA

摘要

PURPOSE: A kit for determining thyrotrophic hormone using radio immunoassay(RIA) is provided, thereby the amount of the thyrotrophic hormone in blood can be rapidly and correctly determined, so that the thyroid gland disorder can be determined. CONSTITUTION: The kit for determining thyrotrophic hormone using radio immunoassay(RIA) comprises (a) a standard thyrotrophic hormone antigen; (b) an isotope-labeled thyrotrophic hormone antigen; and (c) an antibody for the thyrotrophic hormone antigens. The method for determining thyrotrophic hormone comprises the steps of: (a) mixing the standard thyrotrophic hormone antigen, the isotope-labeled thyrotrophic hormone antigen and the antibody for the thyrotrophic hormone antigens to produce a standard sample and simultaneously mixing a testing sample with the isotope-labeled thyrotrophic hormone antigen and the thyrotrophic hormone antibody in a reactor; (b) removing the mixture from the reactor, and determining cpm of each reactor on a gamma scintillation counter; (c) preparing a standard graph using the determined cpm value; and (d) calculating the amount of thyrotrophic hormone in a testing sample using the standard graph.