Human-derived tumor cell proliferation inhibitor

摘要

DNA fragments encoding mouse-derived tumor cell proliferation inhibitors can be used as DNA probes to obtain DNA fragments encoding human-derived novel tumor cell proliferation inhibitors from cDNA libraries derived from human colon cancer cells. An expression plasmid containing a promoter derived from Bacillus brevis bacteria, a signal peptide derived from Bacillus brevis bacteria, and a DNA fragment encoding a human tumor cell proliferation inhibitory factor downstream thereof was constructed, and the Bacillus brevis bacteria were transformed using the obtained By culturing the transformant, new inhibitors can be secreted out of the cells, and it is possible to efficiently produce these new inhibitors.