New 2,5-diketo-di-gluconate reductase and its gene and method for preparing 2-keto-L-glonate using E. coli transformed

摘要

The present invention provides a 2,5-diketo-D-gluconate reductase gene isolated from Escherichia coli and 2-keto-L- using the transformed Escherichia coli. It relates to a method for producing a glonate.;The present invention isolates the yqhE gene, which is a gene of 2,5-diketo-di-gluconate reductase from E. coli, and encodes only a protein of 2,5-diketo-di-gluconate reductase, among the genes. Was inserted into the plasmid to prepare a recombinant plasmid pTY-YQH.E. Coli ER2566 (pTY-TQH) was obtained by transfecting E. coli DH5α with the recombinant plasmid, followed by culturing the transformed E. coli ER2566 (pTY-TQH). There is an excellent effect of mass production of 5-diketo-di-gluconate reductase.