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RECOMBINANT VACCINIA VIRUS FOR PRODUCING HEPATITIS C VIRUS COAT PROTEIN 2 AND EXPRESSION THEREOF IN AN ANIMAL CELL
RECOMBINANT VACCINIA VIRUS FOR PRODUCING HEPATITIS C VIRUS COAT PROTEIN 2 AND EXPRESSION THEREOF IN AN ANIMAL CELL
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机译:用于生产丙型肝炎病毒外壳蛋白2的重组痘苗病毒及其在动物细胞中的表达
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PURPOSE: A recombinant vaccinia virus for producing hepatitis C virus coat protein 2 and the expression thereof in an animal cell are provided for development of a hepatitis C vaccine. CONSTITUTION: A recombinant vaccinia virus for producing hepatitis C virus coat protein 2 contains a gene encoding the Korean hepatitis C virus coat protein 2, wherein the Korean hepatitis C virus coat protein 2 gene is inserted within the TK gene of the wild vaccinia virus DNA, and the recombinant vaccinia virus is produced by transforming a mammal cell line with a cloning vector for recombinant vaccinia virus containing the Korean hepatitis C virus coat protein 2 gene together with the wild vaccinia virus DNA; the cloning vector for recombinant vaccinia virus is pMJ IL2-E2; the animal cell line is CV-1 cell line(ATCC CCL70); and the recombinant vaccinia virus is VVLUC-IL2E2(ATCC VR2435). A method for producing the Korean hepatitis C virus coat protein 2 comprises transfection an animal cell line with the recombinant vaccinia virus, wherein the animal cell line is HeLa S3 cell line(ATCC CLL 2.2).
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