Determining optimal hybridization parameters for nucleic acid, useful for detecting immunoregulatory genes, comprises depositing control and test RNA on same membrane

摘要

Determining nucleic acid hybridization parameters using RNA (I) of activated and non-activated macrophage-like cells, fibroblasts or other immune-competent cells, immobilized on a membrane, is new. Determining nucleic acid hybridization parameters using RNA (I) of activated and non-activated macrophage-like cells, fibroblasts or other immune-competent cells, immobilized on a membrane. The new feature is that total RNA (Ia) from an activated macrophage-like cell line, with minimal or compressed gene expression kinetics, and purified mRNA and purified rRNA are fixed to a single membrane, e.g. of nylon, and the parameters determined specifically with labeled RNA or DNA probes. Independent claims are also included for the following: (1) Test membrane for induced gene control Northern blotting comprises a nylon or nitrocellulose membrane on which have been deposited, sequentially, negative and positive controls of a compressed total RNA-kinetic activated cell or tissue, and purified rRNA and purified polyA RNA; and (2) System for induced gene control Northern blotting that includes the membrane of (1).