A method for the separation and highly pure isolation of low molecular weight, structurally similar compounds, in particular of tetra hydro pyrimidine derivatives, such as, for example, 1,4,5,6 - tetrahydrofuran - 2 - methyl - 4 - pyrimidinecarboxylic acids (i) and the cleavage products

摘要

The present invention relates to the separation and highly pure isolation of low molecular weight, structurally similar compounds, in particular of tetra hydro pyrimidine derivatives, such as, for example, 1,4,5,6 - tetrahydrofuran - 2 - methyl - 4 - pyrimidinecarboxylic acids (i) and the cleavage products. These compounds are also in a highly concentrated form and are compatible with the invention are distinguished in that they stabilize under different stress conditions biomolecules. Dollars a at its biotechnical production are always derivative mixtures of different composition obtained from the microorganisms. By means of chemical modification, for example, alkaline hydrolysis, of ectoines as cleavage products are mixtures of different amino acids obtained. Dollars a by the use according to the invention of discontinuous or continuous chromatographic method or the fractional crystallization or a coupling of this method, these components are separately and up to a purity of = 99,9% are obtained. According to the invention, the separations without or with a greatly reduced use of organic solvents are carried out.