Determination of nucleic acids by pcr, measurement of the number of microbial cells, genes or gene copies by means of pcr and a kit for their use

摘要

A PCR amplification product is detected, in quantitative determination of nucleic acid and measurement of the number of bacterial cells or specific genes, by addition of a dye compound which does not fluoresce in the free state but fluoresces in the bonded state to a double-stranded nucleic acid.