Gene null of human liver cancer cell origin

摘要

PURPOSE: To obtain a new DNA useful for the preparation of a proliferation factor originated from human hepatic cancer cell. ;CONSTITUTION: A DNA coding for a proliferation factor originated from human hepatic cancer cell and having the amino acid sequence of formula. The proliferation factor DNA originated from human hepatic cancer cell can be prepared by extracting mRNA from cultured HuH-7 cell originated from human hepatic cancer cell, synthesizing a double-stranded cDNA from the mRNA, amplifying a DNA fragment of a proliferation factor originated from human hepatic cancer cell by PCR method to design a probe of oligonucleotide for screening, integrating the cDNA into a vector DNA for procaryote to obtain a cDNA library and screening the probe for screening.;COPYRIGHT: (C)1994,JPO