Apparatus and method for multianalyte homogeneous fluorescence immunoassay

摘要

(57) I discloses a method and apparatus of the [summary] fugitive light fluorescence immunoassay. Using a flat waveguide with a (502) semi-cylindrical lens that is integrated (124), multi-analyte and which has a characteristic of the calibration, the apparatus is improved evanescent light field intensity . It is disposed adjacent reservoir of the single (660) in analyte differently to (210) each capture molecule specific preferred embodiment of the assay method and biosensors (120), (720) I have a patch of (662). Capture molecules (720), allowed to bind site-specifically light affinity cross-linking agent (706) a thiol group capture molecules (720) on the waveguide surface (700) surface or the crosslinking agent (700) by being bonded to the coating of specific binding on and fixed patch waveguide surface in (662). Patches of different antibodies to (662,664,666), when the method of binding the (706) Light affinity cross-linking agent, (712), a laser light source mask, the portion of the waveguide surface (700) and (714) It is produced by irradiating selectively using the like.