UDP- glucose: Screening of glycoprotein gluco sill transferase (UGGT) activity

摘要

(57) Abstract This invention regards the method of deciding the effect for the UGGT activity of the test sample. As for this method, a) the test sample and under the existing of UGGT, the accepter substrate of UGGT like acid phosphatase the process which to the sign donor like UDP-3H-GURUKOSU is disclosed; And b) the process which detects the taking in quantity of the donor who spreads to the accepter substrate is included, here, increase of the donor taking in which is compared to contrast means the fact that the test sample is the UGGT stimulant, decrease means the fact that the test sample is the UGGT inhibiter. In addition this invention, it rearranges with cDNA of the mammal which rut/rat UGGT the cord/code does and is isolated and, uses the vector and it regards the method of producing mammal UGGT.