Screening for UDP-glucose: glycoprotein glucosyltransferase (UGGT) activity

摘要

Method for determining the effect of a test sample on UGGT activity comprising the steps of: a) exposing an acceptor substrate of UGGT such as acid phosphatase to a labelled donor such as UDP-3H-glucose in the presence of the test sample and UGGT; and b) detecting the amount of donor which was transferred to the acceptor substrate wherein a decrease of donor intake when compared to a control means that the test sample is a UGGT stimulator and a decrease means that the test sample is a UGGT inhibitor. Also described is an isolated mammalian cDNA that encodes for rat UGGT and to methods of producing mammalian UGGT using recombinant vectors.