High efficiency agurobakuteria germ mediation transformation of the cotton which uses the stem transplantation piece

摘要

(57) Abstract The method of producing the toransujienitsuku cotton plant body with the agurobakuteria germ mediation transformation of stem organization is disclosed. As for this method, the below-mentioned process: (A) the cotton stem transplantation piece is acquired, (b) the stem transplantation piece, the contact obtaining, the agurobakuteria germ is something which can transport to the stem transplantation one cell genome of the exogenous gene and the selective physical system gene stably in the culture medium of the agurobakuteria germ Agrobacterium tumefaciens which keeps the exogenous gene and the vector which includes the selective possible marker, (c) the stem transplantation piece is cultured and callus formation is induced, (d) the transformation callus which reveals the exogenous gene is selected, (e) the callus which is selected is cultured in the suspension nutrient medium and formation of the embryoid is induced, (f) the embryoid playback the fact that it points is included in all the toransujienitsuku cotton plant bodies.