NUCLEIC ACID ANALOGUE AND ITS USE IN DIAGNOSTICS AND ANALYTICAL PROCEDURE

摘要

PPROBLEM TO BE SOLVED: To provide nucleic acid analogues for use in the capture, recognition, detection, identification, or quantification of one or more chemical or biological entities, and to provide methods for using the analogues in diagnostics and analytical procedures. PSOLUTION: The nucleic acid analogue is (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along the backbone, the ligands being each independently naturally-occurring nucleobases, non- naturally-occurring nucleobases or nucleobase-binding groups, each ligand being bound directly or indirectly to a nitrogen atom in the backbone, and the ligand bearing nitrogen atoms mainly being separated from one another in the backbone by 4 to 8 intervening atoms, (b) a nucleic acid analogue capable of hybridizing to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to the analogue and the nucleic acid, or (c) a nucleic acid analogue capable of hybridizing to double-stranded nucleic acid in which one strand has a sequence complementary to the analogue, so as to displace the other strand from one strand. The nucleic acid analogue is preferably a compound of the formula (wherein, L is selected from H, phenyl and the group consisting of monocyclic, bicyclic or tricyclic hererorings, naturally-occurring nucleobases and non-naturally-occurring nucleobases; R7' is selected from H and the group consisting of naturally-occurring alpha-amino acids' side chains; n is an integer of 1-60; k, l and m are each 0 or an integer of 1-5; preferably, k+m=1 or 2, most preferably 1; Rh is OH, NH2 or NHLysNH2; and Ri is H or COCH3). PCOPYRIGHT: (C)2003,JPO