ENZYME IMMOBILIZATION METHOD AND IMMOBILIZED ENZYME MEMBRANE

摘要

PROBLEM TO BE SOLVED: To solve the problem of a conventional immobilized enzyme membrane having a high degree of technical difficulty wherein the immobilization of a target enzyme and the integration of the membrane usually having three phases are complicated and difficult.;SOLUTION: This enzyme immobilization method comprises introducing a silane coupling agent into a polymer membrane which is a phase on a specimen side, and then binding the target enzyme to the functional group of the introduced silane coupling agent through a divalent reagent, or the like. The immobilized enzyme membrane is prepared by sprinkling a different or identical polymer dissolved in a solvent on the enzyme-immobilized phase side to form and integrate the thin membrane.;COPYRIGHT: (C)2003,JPO