Electrospun Poly(acrylonitrile-co-acrylic acid) Nanofibrous Membranes for Catalase Immobilization:Effect of Porphyrin Filling on the Enzyme Activity

摘要

Porphyrin-filled nanofibrous membranes were facilely prepared by electrospinning of the mixtures of poly(acrylonitrile-co-acrylic acid)(PANCAA) and porphyrins. 5,10,15,20-Tetraphenylporphyrin(TPP) and its metal-loderivatives(ZnTPP and CuTPP) were studied as filling mediators for the immobilization of redox enzyme. Results indicate that the introduction of TPP, ZnTPP and CuTPP improves the retention activity of the immobilized catalase. Among these three porphyrins, the ZnTPP-filled PANCAA nanofibrous membrane exhibits an activity retention of 93%, which is an exciting improvement. This improvement is attributed to both the strong catalase-porphyrin affinity and the possible facilitated electron transfer induced by the porphyrin as evidenced by quartz crystal microbalance (QCM) and fluorescence spectroscopy studies.