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Enzyme/chemical reactor based protein processing method for proteomics analysis by mass spectrometry
Enzyme/chemical reactor based protein processing method for proteomics analysis by mass spectrometry
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机译:基于酶/化学反应器的蛋白质组学蛋白质组学分析方法
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摘要
A method and tools for the biochemical processing of protein samples towards their analysis and identification by, for example, mass spectrometry (MS). The method involves the reversible (non-covalent) immobilization, purification and concentration of proteins onto a solid surface and subsequent solid-phase enzyme-catalyzed proteolysis of the proteins. The constituent peptides are recovered in near-quantitative yields in a format ideally suited for identification by routine MS technologies. The process is easily adapted such that additional chemical and/or enzymatic transformation(s) of the immobilized proteins or peptides can be performed to facilitate the acquisition of and increase the information content obtained from the MS analysis.
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