Gene expression alterations underlying the retardation of aging by caloric restriction in mammals

摘要

A method of measuring the relative metabolic state of a multicellular organism is disclosed. In one embodiment, the method comprises the steps of: (a) obtaining a sample of nucleic acid isolated from the organism's organ, tissue or cell, wherein the nucleic acid is RNA or a cDNA copy of RNA, (b) determining the gene expression pattern of at least one of the genes selected from the group consisting of D31966, R74626, U79163, M22531, U43285, U79523, X81059, X84239, D38117, M70642, U37775, U84411, D87117, U31966, U51167, M97900, U32684, U43836, U60001, X61450, D49473, L08651, U28917, U49507, X59846, X00958, K03235, Z48238, M60596, AA117417, AF007267, AF011644, AJ001101, C79471, D16333, D49744, D83146, D86424, L29123, L40632, M74555, M91380, M93428, U19799, U20344, U34973, U35312, U35646, U43512, U47008, U47543, U56773, X06407, X54352, X84037, Y00746, Y07688, Z19581, Z46966, AF003695, AF020772, C76063, C79663, D10715, D12713, D67076, D86344, L10244, L18888, M57966, M58564, U19463, U25844, U27830, U35623, U43892, U51204, U75321, U84207, X52914, X54424, X75926, X99921 and Z47088 and (c) determining whether the gene expression profile of step (b) is more similar to a CR-induced metabolic state or a standard diet metabolic state.