GENE EXPRESSION ALTERATIONS UNDERLYING THE RETARDATION OF AGING BY CALORIC RESTRICTION IN MAMMALS

摘要

Disclose a kind of method for measuring the relative metabolic state an of multicellular organism. In one embodiment,Method includes the following steps::(a)From the tissue of organism,Tissue or unit obtain a sample of nucleic acid isolataed,Wehrein nucleic acid is the RNA or complementary DNA copy of RNA,(b)It determines selected from by D31966,R74626 ,U79163 ,M22531 ,U43285 ,U79523 ,X81059 ,X84239 ,D38117 ,M70642 ,U37775 ,U84411 ,D87117 ,U31966 ,U51167 ,M97900 ,U32684 ,U43836 ,U60001 ,X61450 ,D49473 ,L08651 ,U28917 ,U49507 ,At least one gene expression pattern of the gene of group of X59846 compositions,X00958 ,K03235 ,Z48238 ,M60596 ,AA117417 ,AF007267 ,AF011644 ,AJ001101 ,C79471 ,D16333 ,D49744 ,D83146 ,D86424 ,L29123 ,L40632 ,M74555 ,M91380 ,M93428 ,U19799 ,U20344 ,U34973 ,U35312 ,U35646 ,U43512 ,U47008 ,U47543 ,U56773 ,X06407 ,X54352 ,X84037 ,Y00746 ,Y07688 ,Z19581 ,Z46966 ,AF003695 ,AF020772 ,C76063 ,C79663 ,D10715 ,D12713 ,D67076 ,D86344 ,L10244 ,L18888 ,M57966 ,M58564 ,U19463 ,U25844 ,U27830 ,U35623 ,U43892 ,U51204 ,U75321 ,U84207 ,X52914 ,X54424 ,X75926 ,X99921 and Z74088 and(c),It determines whether step(b)Gene expression profile be more closely similar to a CR induction metabolic state or a standard diet metabolic state.