首页>
外国专利>
Array based hybridization assays employing enzymatically generated labeled target nucleic acids and compositions for practicing the same
Array based hybridization assays employing enzymatically generated labeled target nucleic acids and compositions for practicing the same
展开▼
机译:基于阵列的杂交测定法,采用酶促生成的标记靶核酸及其组合物进行操作
展开▼
页面导航
摘要
著录项
相似文献
摘要
Methods for assaying a sample for the presence of one or more nucleic acid analytes, either qualitatively or quantitatively, are provided. In the subject methods, an array of DNA primers is contacted with the sample being assayed under conditions sufficient to produce labeled target nucleic acids enzymaticallyon the surface of the array at locations where a DNA primer has hybridized to a nucleic acid analyte to produce a duplex nucleic acid. The presence of labeled target nucleic acids on the array surface is then detected and used to determine the presence of the one or more nucleic acid analytes in the sample. Also provided are kits for practicing the subject methods. The subject invention finds use in a variety of different applications, including differential gene expression analysis.
展开▼