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Maxam gilbert g/a sequence analysis by DHPLC
Maxam gilbert g/a sequence analysis by DHPLC
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机译:Maxam Gilbert g / a通过DHPLC进行序列分析
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摘要
The present invention describes a method for generating a chromatographic DNA sequencing ladder useful for phasing a chromatographic separation of fragments derived from the DNA sequence, e.g., a DNA footprinting reaction. The chromatographic separation is preferably accomplished using ion pairing reverse phase high performance liquid chromatography under conditions that are substantially free of multivalent cations that are free to interfere with polynucleotide separations.
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