Method for increasing levels of export of mRNA from the nucleus to the cytoplasm by providing in the nucleus recombinant mammalian export protein ALY

摘要

The present invention features methods for increasing levels of nuclear export of mRNA by providing recombinant mammalian export factor. Also featured are methods for preparing metazoan or mammalian cells with recombinant factor ALY that are useful for facilitating mRNA export. The export factor ALY binds mRNA in a non sequence specific manner and the ALY-mRNA complex is exported more efficiently than unbound mRNA. The addition of excess ALY to metazoan or mammalian cell results in increased mRNA export, increased gene expression and increased production of recombinant proteins.