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Positive identification of phospho-proteins using motif-specific, context-independent antibodies coupled with database searching
Positive identification of phospho-proteins using motif-specific, context-independent antibodies coupled with database searching
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机译:使用基序特异性,上下文无关的抗体和数据库搜索对磷酸化蛋白进行阳性鉴定
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摘要
The invention provides a method for producing antibodies that selectively recognize short, modified amino acid motifs substantially independent of the surrounding amino acid context in which the motif occurs. A novel class of motif-specific, context-independent antibodies is also provided. The invention encompasses modified motifs consisting of single modified amino acids, for example phosphotyrosine or acetylated lysine, as well other modified motifs of multiple amino acids, such as kinase consensus substrate motifs and protein-protein binding motifs relevant to cell signal transduction. Also provided are methods of profiling large and diverse protein populations on a genome-wide basis by utilizing the antibodies of the invention, and methods for the positive identification of cellular phosphoproteins using one or more motif-specific, context-independent antibodies of the invention coupled with protein database searching.
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