POSITIVE IDENTIFICATION OF PHOSPHO-PROTEINS USING MOTIF-SPECIFIC, CONTEXT-INDEPENDENT ANTIBODIES COUPLED WITH DATABASE SEARCHING

摘要

The present invention is related to a method for producing motif-specific, context-independent antibodies which are specific to at least one modified fixed amino acid residue in the context of variable surrounding amino acid or peptide sequences. The method is particularly useful in producing antibodies which recognize phosphorylated serine, threonine, and tyrosine, or acetylated lysine, as well as other modified amino acid-containing motifs of one or more amino acids.