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Transgenic plants expressing a dual-specificity MAPK phosphatase and uses thereof

机译:表达双重特异性MAPK磷酸酶的转基因植物及其用途

摘要

Disclosed are transgenic plants which include a transgene which expresses a phosphatase domain of a dual-specificity mitogen-activated protein kinase (MAPK) phosphatase, wherein the transgene is expressed in the transgenic plant under the control of a promoter that is functional in a plant cell. Also disclosed are methods which are useful for modifying the phenotype of a plant, including the steps of: (a) introducing into a plant cell a transgene comprising DNA encoding a phosphatase domain of a dual-specificity mitogen-activating protein kinase (MAPK) phosphatase operably linked to a promoter functional in plant cells to yield a transformed plant cell; and (b) regenerating a transgenic plant from the transformed cells, wherein the phosphatase domain of the dual-specificity MAPK phosphatase is expressed in the cells of the transgenic plant, thereby modifying the phenotype of the transgenic plant.
机译:公开了转基因植物,其包括表达双特异性有丝分裂原活化蛋白激酶(MAPK)磷酸酶的磷酸酶结构域的转基因,其中所述转基因在转基因植物中在植物细胞中起作用的启动子的控制下表达。 。还公开了可用于修饰植物表型的方法,包括以下步骤:(a)将转基因导入植物细胞,该转基因包含编码双特异性促细胞分裂原活化蛋白激酶(MAPK)磷酸酶的磷酸酶结构域的DNA。与在植物细胞中起作用的启动子可操作地连接以产生转化的植物细胞; (b)从转化的细胞再生转基因植物,其中双特异性MAPK磷酸酶的磷酸酶结构域在转基因植物的细胞中表达,从而修饰转基因植物的表型。

著录项

  • 公开/公告号US6548743B1

    专利类型

  • 公开/公告日2003-04-15

    原文格式PDF

  • 申请/专利权人 THE GENERAL HOSPITAL CORPORATION;

    申请/专利号US19990371671

  • 发明设计人 JEN SHEEN;WAN-LING CHIU;

    申请日1999-08-10

  • 分类号A01H50/00;A01H51/00;C12N50/40;C12N158/20;C12N155/40;

  • 国家 US

  • 入库时间 2022-08-22 00:07:07

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