Se36 polypeptide, se36 polypeptide purification process, malaria vaccine, malaria diagnostic agent and synthetic DNA fragment

摘要

"SEP POLYPEPTID, PROCESS FOR PURIFICATION OF SE36 POLYPEPTIDE, MALARIA VACCINE, MALARIA DIAGNOSIS AND SYNTHETIC DNA FRAGMENT AGENT". The present invention provides an SE36 polypeptide derived from the sera N-terminal domain (47 kd) of sera (serine repeat antigen) produced by the malaria parasite Plasmodium falciparium at the erythrocyte stage; a process for purifying it; and a malaria vaccine and a diagnostic agent using said purified antigen thus obtained as an active ingredient. By deleting all or part of the polymerized serine from the 47 kd serine repeat domain, a large amount of SE36 is produced by Escherichia coli and can be purified to a high extent. Human IgG3 antibodies specifically bind to SE36, greatly and effectively preventing parasite proliferation in erythrocytes and consequently inhibiting fever and cerebral malaria, thereby preventing death.