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INTRODUCTION OF A THIOL GROUP IN PROTEINS FOR RADIOIMUN DETECTION AND RADIOIMMUNTHERAPY BASED ON RADIONUCLIDES
INTRODUCTION OF A THIOL GROUP IN PROTEINS FOR RADIOIMUN DETECTION AND RADIOIMMUNTHERAPY BASED ON RADIONUCLIDES
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机译:在基于放射性核素的放射性免疫检测和放射免疫治疗中引入巯基基团
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摘要
A method of radiolabeling a protein with a radionuclide including contacting the protein with a protected tertiary thiol-containing bifunctional chelating agent that is capable of reacting with the protein at one end of the agent and is capable of complexing with a radionuclide at the other end of the agent, to form a protein-acetyl-t-thiol-containing conjugate. The protein-acetyl-t-thiol-containing conjugate then is deprotected and admixed with a reducing agent for the radionuclide, where the radionuclide is added in a subsequent step, to form a mixture of reducing agent and protein-t-thiol-containing conjugate. This mixture then is reacted with a radionuclide whereby the radionuclide reacts with pendant sulfhydryl groups present on the protein-t-thiol-containing conjugate. Methods of radioimmunotherapy and diagnostic kits suitable for forming a composition to be administered to a human patient also are disclosed.
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