New isolated, enriched, cell free and/or recombinant nucleic acid useful for e.g. altering cell proliferation characteristic such as to alter plant cell, organ or tissue size

摘要

An isolated, enriched, cell free and/or recombinant nucleic acid (II) comprising a sequence encoding for expression of a protein or peptide (I) capable of altering E2F-dimerization partner (DP) activity in a plant cell is new. (P1) is characterized in that it has one or both DP activities in plants selected from: (1) the ability to dimerized with plant E2F protein; and (2) the ability to modulate, particularly enhance, E2F binding to E2F transcription factor binding sites in plant DNA or effect of it characterized in that the protein or peptide comprises a 261 amino acid sequence (S1) or a functionally active part of it or a sequence having at least 70% homology to so the sequence or part of it. Independent claims are also included for the following: (1) controlling one or more of plant growth, gene expression, cellular DNA replication, cell cycle progression, differentiation and development comprising increasing or decreasing DP protein activity in a plant cell through the expression of (P1); (2) isolated (I); (3) a nucleic acid probe (III) comprising a DNA sequence corresponding to an amino acid sequence comprising 261, 458, 52, or 51 amino acids fully defined in the specification; (4) a nucleic acid probe or primer (IV) comprising a double or single stranded DNA of sequence corresponding to 10 or more contiguous nucleotides taken from a sequence (S2) comprising 1089 base pairs fully defined in the specification, with the proviso that it is not selected from residues 70-136; (5) an oligonucleotides probe (V) comprising at least 18 contiguous bases of S2; (6) an antisense DNA (VI) to (II), (III), (IV) or (V); (7) a nucleic acid vector or construct (VII) comprising (II), (III), (IV), (V), or (VI) or its antisense nucleic acid; (8) a plant cell (IX) comprising (VIII); (9) a transgenic plant or its part (X) comprising (IX); (10) an antibody (XI) raised against (I); (11) identifying and/or isolating (M2) DNAs corresponding to complete or partial genes that are regulated in G1 passage, G1/S phase transition and/or S phase progression of the cell cycle, involves contacting sample of genomic DNA with binding material specific for binding such complete or partial genes, removing non-bound DNA from the specific binding material, and releasing and isolating the bound DNA, where the specific binding material comprises a peptide or protein including the DNA binding sequence of a protein that is capable of acting as a part of a plant hetero-oligomer transcription activator or repressor; and (12) a specific binding material (XII) which comprises a peptide or protein having DNA binding activity with respect to plant DNA transcription activator or repressor factor binding sites, particularly in genomic DNA, and having the ability to dimerize or oligomerize with a further such plant protein together with one or more of a further peptide or protein.