Isolated nucleic acid and antibody molecule, genetic construction, processes to increase the level of cellulose, non-crystalline beta-1,4-glucan and starch and to reduce the level of non-crystalline beta-1,4-glucan, to starch and cellulose in a cell, tissue, organ or organism, to produce an enzymatically active recombinant polypeptide and to alter the mechanical properties of a cell wall, recombinant polypeptide, transgenic plant and use of an isolated nucleic acid molecule.

摘要

"ISOLATED AND ANTIBODY NUCLEIC ACID MOLECULE, GENETIC CONSTRUCTION, PROCESSES TO INCREASE THE LEVEL OF NON-CRYSTALLINE BETA-1,4-GLUCAN AND STARCH AND TO REDUCE THE NON-CHRISTIAN, BETA-1,4-GLUCAN LEVEL OF STARCH AND CELLULOSE IN A CELL, TISSUE, ORGAN OR ORGANISM, TO PRODUCE A RECOMBINANT, ENZYMATICALLY ACTIVE POLYPEPTIDE AND TO CHANGE THE MECHANICAL PROPERTIES OF A CELLULAR WALL, NON-RECOMBINATING MOLE WALL. Sequences of cDNA and polypeptides having diacylglycerol CDP synthase (CDS) enzyme activity are described. CDS is also known as CTP: phosphatidate cytidyltransferase. Also described are processes for isolating and producing polypeptides involved in phosphatidic acid metabolism and signaling in mammalian cells, particularly the production of purified forms of CDS.