IN VITRO PRODUCTION OF DENDRITIC CELLS FROM CD14+ MONOCYTES ESPECIALLY FOR THE PREPARATION OF SUSPENSION, MONOLAYER AND THREE-DIMENSIONAL CELL AND/OR TISSUE MODELS, AND USE OF THESE MODELS

摘要

The invention relates to the use of CD14+ monocytes for the production ofdendritic cells. The invention comprises the use of CD14+ monocytes isolatedfrom peripheral circulating blood for obtaining, by differentiation, at leastone mixed population of Langerhans cells and interstitial dendritic cells,both Langerhans cells and interstitial dendritic cells being preconditionedand undifferentiated, and/or differentiated and immature, and/or mature,and/or interdigitated. The invention comprises their use in suspension,monolayer and three-dimensional cell and tissue models. The inventioncomprises the use of these cells and of these models as study models for theassessment of immunotoxicity/immunotolerance, for the development of cosmeticand pharmaceutical active principles and for the development andimplementation of methods of cell and tissue therapy.