METHODS AND COMPOSITIONS FOR DIRECTED MOLECULAR EVOLUTION USING DNA-END MODIFICATION

摘要

Methods as depicted in figure 3, for directed evolution are described where genetic elements are randomly cleaved to permit the deletion or addition of polynucleotides or both to create a library of related genetic elements with additions or deletions. Corresponding library populations are also described. These processes allow a significant sampling of sequence space which is necessary for directed evolution of genes. Further described are methods for effecting very small nucleotide deletions in genetic elements of interest.