BACTERIAL ISOLATES OF THE GENUS KLEBSIELLA, AND AN ISOMALTULOSE SYNTHASE GENE ISOLATED THEREFROM

摘要

The novel bacterial type of two bacterial strains, Klebsiella singaporensis LX3 and LX21 requirement. The novel isomaltulose synthase of the nucleotide sequence (coding) of starting point, KIS are claim. A kind of method is also claimed,Method for producing different plant, including introduce battery the enzyme that is encoded of this plant nucleic acid sequence its converted sucrose and allow the cell in such a way to isomaltulose to express the nucleic acid sequence, and the method for the coding nucleotide sequence of functional cloning method separation KIS albumen, gene pool, which is prepared, including (one) includes a kind of DNA sequence dna from donor organism, encode a kind of active suitable host organism of isomaltulose biosynthesis, b) screening and cloning gene pool is by the reducing sugar content enhanced by it, (3) separation clone contains a coding protein and isomaltulose biosynthesis activity.