首页> 外国专利> ATP REGENERATION REACTION SYSTEMS AND METHOD OF EXAMINING ADENINE NUCLEOTIDE, METHOD OF DETECTING RNA AND METHOD OF AMPLIFYING ATP BY USING THE

ATP REGENERATION REACTION SYSTEMS AND METHOD OF EXAMINING ADENINE NUCLEOTIDE, METHOD OF DETECTING RNA AND METHOD OF AMPLIFYING ATP BY USING THE

机译:ATP再生反应系统和检查腺嘌呤核苷酸的方法,检测RNA的方法和使用该酶扩增ATP的方法

摘要

The present invention relates to an ATP-regeneration reaction system comprising the steps of acting adenylate kinase on AMP to convert it into ADP and acting a polyphosphoric acid synthetase in the presence of a polyphosphoric acid compound to convert it into ATP and a polyphosphoric acid compound; an ATP-regeneration reaction system comprising the steps of acting a phosphotransferase on AMP in the presence of a polyphosphoric acid compound to convert it into ADP and then acting a polyphosphoric acid synthetase on the resulting ADP to convert it into ATP; and a method for detecting or inspecting adenine nucleotide or RNA and an ATP-amplification method, which make use of the foregoing regeneration system.
机译:本发明涉及一种ATP再生反应系统,该系统包括以下步骤:在AMP上作用腺苷酸激酶以将其转化为ADP,以及在多磷酸化合物存在下作用于多磷酸合成酶以将其转化为ATP和多磷酸化合物。 ATP再生反应系统,其包括以下步骤:在多磷酸化合物存在下,使磷酸转移酶对AMP起作用,以将其转化为ADP,然后对所得的ADP起作用,使多磷酸合成酶将其转化为ATP。利用上述再生系统的腺嘌呤核苷酸或RNA的检测方法及ATP扩增方法。

著录项

  • 公开/公告号EP1264894A1

    专利类型

  • 公开/公告日2002-12-11

    原文格式PDF

  • 申请/专利权人 SATAKE CORPORATION;

    申请/专利号EP20010901364

  • 发明设计人 KURODA AKIO;OHTAKE HISAO;TANAKA SHOTARO;

    申请日2001-01-17

  • 分类号C12P19/32;C12Q1/48;C12Q1/66;C12Q1/68;

  • 国家 EP

  • 入库时间 2022-08-21 23:54:39

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