Method of cultivation, attenuation of virulence and the in vitro cloning of the genus Babesia and their applications

摘要

The invention relates to processes for the culture, attenuation of the virulence and cloning of the kind in vitro of parasites babesia, parasites of the attenuated virulence and the clones of the type babesia, nucleotide probes derived from the dna of the clones, to the live attenuated vaccines against the babesiosis and to the applications of said methods. & br / the process of in vitro culture comprises at least one red blood cells, the incubation of parasitised by the parasites in the presence of red blood cells are not parasitised in a medium and the suitable culture conditions and is characterized in that the incubations are performed in the presence of cells capable of acting as support for the culture of the parasites. & br / the process of the attenuation in vitro the virulence of the parasites comprises the culture of the parasites, for at least eight days, while the process for the cloning, in vitro, comprises the isolation of a single parasitic and the culture of the latter, these operations can be repeated.