Culture methods of attenuation of virulence and in vitro cloning of parasites of the genus Babesia and applications.

摘要

The invention APPROACHING culture processes, DE Attenuation of virulence AND CLONING IN VITRO Parasitic TYPE BABESIA, A Parasite Virulence tempered with CLONES OF TYPE BABESIA, Sonda Nucleotide obtained from DNA OF SUCH CLONES, A VACCINE VIVAS THE BABEOVIOSIS attenuated and applications of these processes. THE PROCESS OF CULTURE IN VITRO comprises at least one incubation of erythrocytes parasitized by these parasites IN THE PRESENCE OF RBCs homologues No parasitized IN A MEDIUM AND CONDITIONS OF SUITABLE CROPS AND IS DEFINED IN THE O incubations PERFORMED IN THE PRESENCE OF CELLS INTENDED TO SERVE SUPPORT CROP OF SUCH PARASITES. THE PROCESS OF DIM IN VITRO Virulence Parasite comprises culturing said parasites FOR AT LEAST EIGHT DAYS WHILE THE PROCESS OF CLONING IN VITRO comprising the isolation of a single parasite and cultivating the latter may be repeated these operations.