Pressure sensitive adhesive sheet for detection of microorganism and method for detection of microorganism

摘要

A pressure sensitive adhesive sheet for the detection of microorganisms, which comprises a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane which does not allow passage of the microorganisms; a pressure sensitive adhesive sheet for the detection of microorganisms, wherein the surface of the adhesive layer has a contact angle with water of not more than 90°; and a method for detecting a microorganism, which comprises bringing the surface of an adhesive layer of a pressure sensitive adhesive sheet into contact with the surface of a test object, and then bringing the surface of the adhesive layer into contact with water, wherein said adhesive layer or water contains a chromogenic reagent.;The use of a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane makes it possible to leave only stained microorganisms on the water-permeable membrane and to precisely observe the color developed by the microorganisms.;The method for detecting microorganisms of the present invention enables real-time monitoring without culture of microorganisms, and is not limited to the detection of viable cells alone. Inasmuch as a contact of the pressure sensitive adhesive sheet with a test face results in accumulation of microorganisms, the manipulation is simple. In addition, by properly determining a chromogenic reagent, a specific microorganism alone can be detected and counted, so that it can be applied to environmental investigation in the medical field, food industry field and the like.