SUSPENSION SYSTEM FOR SEQUENCING GENETIC SUBSTANCE, METHOD OF SEQUENCING GENETIC SUBSTANCE BY USING THE SUSPENSION SYSTEM, AND METHOD OF HIGH-SPEED SNPs SCORING BY USING THE SUSPENSION SYSTEM

摘要

An object in relation to a suspension for determining the sequence of genetic materials, a method of determining the sequence of genetic materials using such a suspension, and a method for high-speed scoring SNPs using such a suspension, is to ensure high reliability, and substantially increase reaction efficiency, to thereby shorten the time until reaching an equilibrium.;The construction is such that the base sequence of a genetic material being a target, is determined using, suspended in a liquid in a container: a first coded oligonucleotide group which includes first coded oligonucleotides having one type of base sequence of a predetermined base number which have been coded so as to identify the base sequence thereof, so as to cover the base sequences of all the types or specific types for the base number thereof, and which are each provided so as to be sortable as one belonging to this group, a second coded oligonucleotide group which includes second coded oligonucleotides having one type of base sequence of a predetermined base number which have been coded so as to identify the base sequence thereof, so as to cover the base sequences of all the types or specific types for the base number thereof, and which are each provided so as to be sortable as one belonging to this group, and an enzyme which differentially bonds the deoxyribose pair being a sugar of the end nucleotide thereof, only in the case of a situation where the two coded oligonucleotides are hybridized to a single strand genetic material and the end nucleotide pair of the base sequence thereof are directly adjacent, and the base sequence of the predetermined base number is a constant relation with the base sequence of that genetic material.