PRODUCTION OF RECOMBINANT CHE A 1 ALLERGEN FROM CHENOPODIUM ALBUM AND SYSTEM OF ISOLATING AND PURIFYING SAME

摘要

The invention relates to the production of the Che a 1 protein, using recombinant technology, in Pichia pastoris yeast which involves the use of nucleotide sequence SEQ ID NO:1 or other nucleotide sequences obtained by mutagenesis of sequence SEQ ID NO:1. For this purpose, a main characterised and purified allergen is isolated from Chenopodium album pollen, said allergen being recognised by more than 60 % of patients allergic to said biological source. In this way, the recombinant DNA which codes for the Che a 1 allergen from goosefoot pollen (Chenopodium album) has been cloned and expressed. Said allergen shows homology with ryegrass and birch proteins. The recombinant protein produced in yeast (Pichia pastoris) is isolated with high yields, is correctly folded and exhibits immunological properties equivalent to those of the natural allergen. Hence, said protein and the homologous proteins thereof, the modified forms derived from same and derived fragments containing at least one allergen epitope can be used in immunotherapy and diagnosis protocols.