Production of recombinant allergen 'Chenopodium album' che 1 and system isolation and purification.

摘要

Production of recombinant allergen "Chenopodium album" Che a 1, and isolation and purification system.; The invention consists in the production of Che protein 1, by recombinant technology in yeast "Pichia pastoris", which involves the use of the nucleotide sequence characterized by SEQ ID NO: 1 or other nucleotide sequences obtained by mutagenesis of the sequence SEQ ID NO: 1. for it has been isolated from pollen "Chenopodium album", purified and characterized a major allergen which is recognized by more than 60% of patients allergic to this biological source. It has also been cloned and expressed the recombinant DNA encoding the allergen Che 1 ceñigo pollen (Chenopodium album). This allergen protein has homology with birch and ryegrass. Recombinant protein produced in yeast (Pichia pastoris) is isolated in high yield is correctly folded and exhibits immunological properties equivalent to those of natural allergen so that it and the homologous proteins, modified forms derived therefrom, derived fragments containing at least one allergenic epitope, they can be used in diagnosis and immunotherapy protocols.