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DETERMINATION OF HYDROPHOBIC COENZYME A ESTERS AND OTHER LIPIDS USING A BIOSENSOR COMPRISING A MODIFIED COENZYME A- AND ACYL-COA BINDING PROTEIN (ACBP)
DETERMINATION OF HYDROPHOBIC COENZYME A ESTERS AND OTHER LIPIDS USING A BIOSENSOR COMPRISING A MODIFIED COENZYME A- AND ACYL-COA BINDING PROTEIN (ACBP)
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机译:含修饰的辅酶A-和酰基-COA结合蛋白(ACBP)的生物传感器测定疏水性辅酶A和其他脂质
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摘要
The invention relates to a biochemical assay for wide class of hydrophobic Coenzyme A esters wherein the analyte is caused to react with a specifically binding, modified protein, and thereby causing a detectable signal. A one step assay for hydrophobic carboxylic acid esters in whole blood, serum, food and feed preparations, tissue extracts, acyl-CoA synthetase reaction media and various laboratory conditions using a modified Coenzyme A- and acyl-CoA binding protein (ACBP) is provided. Furthermore the invention relates to a construct comprising a peptide and a signal moiety for performing an assay, a kit for assaying hydrophobic CoA esters, hydrophobic carboxylic acids, triacylglycerides, phospholipids, and cholesterolesters.
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