COMBINATION OF MICROSATELITE MARKER FOR FINGERPRINTING OF KOREAN-BRED RICE CULTIVARS AND THE SIGNIFICANT CODE NUMBER THEREBY

摘要

PURPOSE: Provided are a microsatellite DNA marker combination for classification of rice varieties and specific code numbers given thereby, therefore each of 148 kinds of Korean rice has a specific code number, so that the Korean rice can be easily classified. CONSTITUTION: The microsatellite DNA marker combination for classification of rice varieties consists of six(6) microsatellite DNA markers of RM48, RM249, RM209, RM70, RM257 and OSR20, wherein they are positioned at the 2nd, 5th, 6th, 7th, 9th and 12th sites in a rice chromosome, respectively, and have their own numbers of two digits according to each PCR amplified allele. Each variety of the rice is given code number of 11 digits by combination of the 6 microsatellite markers according to chromosome numbers; and the microsatellite DNA markers are selected by using PCR amplification and 4% acrylamide electrophoresis.