65 HSP 65 GENE FRAGMENTS AND METHOD OF IDENTIFYING MYCOBATERIAL SPECIES WITH THE SAME

摘要

PURPOSE: An hsp 65 gene fragment of Mycobacterium species and a method for identifying Mycobacterial species using the same are provided, thereby easily and cheaply identifying Mycobacterial species. CONSTITUTION: A polynucleotide representing 604-bp fragment of the hsp 65 gene of Mycobacterium species has the nucleotide sequence selected from SEQ ID NOs: 1 to 54. A nucleotide set for identifying Mycobacterium species comprises at least one 604-bp fragment of the hsp 65 gene of Mycobacterium species selected from SEQ ID NOs: 1 to 54. A method for identifying Mycobacterial species comprises the steps of: (1) amplifying an hsp 65 gene fragment of an interest strain using a primer; (2) analyzing the nucleotide sequence of the amplified hsp 65 gene fragment; and (3) comparing the nucleotide sequence of the hsp 65 604-bp fragment of the interest strain with that of standard Mycobacterium strain.