Regulating gene expression and viral replication by switching off an active mutated promoter of a repressor allows selective expression of therapeutic genes and viral vectors in tumor cells

摘要

Regulating gene expression and viral replication by switching off an active mutated promoter of a repressor, where a tumor-specific gene and viral vector is used where the repressor protein has specific binding ability for a target selectively expressed in degenerate cells, is new. Independent claims are also included for the following: (1) reducing toxicity of viral vectors by expressing a transcriptional repressor, where neutralization of the vector through a p53 binding site in the vector, for example the p53 promoter, is driven by p53; and (2) improved kinetics of viral replication where the virus' own promoters are replaced by stronger CMV promoters.