New method for the determination of the telomere length which and their use for the estimation of the age

摘要

The present invention relates to a method for estimation of the age on the basis of a tissue sample. Furthermore, with the method according to the invention, the statistical life expectancy of people are estimated. Dollars a the new method for measuring the telomere length which, in which the length of telomeric by means of a blot - process and, if appropriate, subsequent hybridization of the to the from the blot - method resulting membrane bound, separated by gel electrophoresis dna with a to the chain telomer sequence complementary nucleic acid - probe, and wherein the chain telomer - dna samples - dna containing in front of the gel electrophoretic separation is subjected to a complete restriction digest, is characterized in that the separation of the undigested dna in the the blot - forming the basis for the process of gel electrophoresis in the case of dollars a (a) & 5 volts / cm and dollars a (b) for the duration of at least 4 hours takes place and dollars a (c) of the detection of the complex of chain telomer - dna and probes - nucleic acid is carried out by means of a color reaction.