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New method for the determination of the telomere length which and their use for the estimation of the age
New method for the determination of the telomere length which and their use for the estimation of the age
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机译:确定端粒长度的新方法及其在年龄估计中的用途
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摘要
A method of assessing the age or expectation of life wherein the length of the telomeres is determined by a blot method and, optionally, by subsequently hybridizing the DNA gel-electrophoretically separated and bound to the membrane obtained through the blot method by means of a nucleic acid probe complementary to the telomeric sequence, and wherein the specimen DNA containing the telomeric DNA prior to the gel-electrophoretic separation is subjected to a full restriction digestion, characterized in that the separation of the digested DNA in the gel-electrophoresis underlying the blot method takes place at (a) 5 V/cm, and (b) for the duration of at least 6 hours.
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机译:一种评估年龄或预期寿命的方法,其中端粒的长度是通过印迹方法确定的,并且任选地,随后通过凝胶电泳分离分离并结合到通过印迹方法获得的膜上的DNA的DNA与端粒序列互补的酸性探针,其中在凝胶电泳分离之前对包含端粒DNA的样本DNA进行完全限制性消化,其特征在于,凝胶电泳中消化的DNA的分离是印迹方法的基础(a)<5 V / cm,(b)至少持续6小时。
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