Pichia pastoris and as recombinant DNA for the transformation thereof an integrative vector which contains a functional selection marker of Saccharomyces cerevisiae, the promotor of the alcohol oxidase gene (AOX₁) of the yeast itself, as the transcription terminator that of glyceraldehyde 3-phosphate dehydrogenase (GAP) of S. cerevisiae, or that which is carried by the heterologous gene to be expressed, and another sequence homologous to the genome of Pichia pastoris which serves for the integration thereof. The expression vectors used further contain a heterologous gene bound to the signal peptide of the sucrose invertase gene (SUC2) of S. cerevisiae. This method guarantees that the polypeptide products are recoverable under controllable conditions and with high yields. The strains according to the present invention can be used for the large-scale production of pharmaceutical products or of enzymes of industrial interest."/>
公开/公告号DE69133066T2
专利类型
公开/公告日2003-03-06
原文格式PDF
申请/专利号DE1991633066T
发明设计人 PAIFER REYES EDENIA;FERBEYRE BINELFA GERARDO;S OSA ESPINOSA ANGELA ESTELA;HERRERA MARTINEZ LUIS SATURNINO;GONZALEZ LŸPEZ TANIA;MENENDEZ ALARCŸN ALFREDO;DELGADO BOADA JULIO MARCOS;TORRENS MADRAZO ISIS DEL CARMEN;AGUIAR SANTIAGO JORGE AGUSTIN;VILLAREAL BARRIOS ADELAIDA;YONG GONZALEZ VLADIMIR;SERALENA MENENDEZ ALINA;SILVA RODRIGUEZ ALEJANDRO;GONZALEZ BADILLO BEATRIZ;MARGOLLEZ CLARK EMILIO;MORALES GRILLO JUAN;MONTESINOS SEGUI RAQUEL;MARTINEZ SANTANA VLADIMIR;CREMATA ALVAREZ JOSE ALBERTO;
申请日1991-01-16
分类号C12N15/81;C12N15/16;C12N15/56;C12N15/57;C12N1/16;
国家 DE
入库时间 2022-08-21 23:39:56