Method of heterologous gene expression in yeast Pichia pastoris The expression vectors and transformed microorganisms.

摘要

The present invention provides a method highly effective for the expression of heterologous genes, using as HUESPED an auxotrophic MUTANTE HIS-3 BKM-90 strain of "Pichia pastoris" And as DNA recombinant for its transformation, an integrative vector containing a selection marker FUNCTIONAL "Saccharomyces cEREVISIAE" ALCOHOL OXIDASE GENE pROMOTER (AOX SUB 1) OF YEAST OWN AS THE tRANSCRIPTION determiner glyceraldehyde 3-phosphate dehydrogenase (GAP) "S. cerevisiae", O THE LEADING THE heterologous gene will express, and other homologous sequence the genome of "Pichia pastoris" SERVE FOR THE iNTEGRATION OF THE SAME. THE USED SUBSEQUENTLY EXPRESSION VECTORS CONTAINING THE KINGDOM heterologous gene indicated peptide gene "SUCROSE INVERTASE" (SUC2) from "S. cerevisiae". This method ensures that POLYPEPTIDE PRODUCTS ARE CONTROLLED CONDITIONS AND recoverable in high yields. STRAINS OF THIS INVENCIONPUEDEN be used for large-scale production PHARMACEUTICAL PRODUCT OR INTERESTING INDUSTRIAL ENZYMES.