Monitoring yeast physiology through fermentations by frequent analyses of gene expression by the TRAC method

摘要

Yeast adapts its physiology to changing conditions during brewery fermentations and handling mainly by transcriptional control. We followed expression of >70 genes through very high gravity fermentations by TRAC (TRanscript analysis with aid of Affinity Capture), which facilitates frequent and accurate transcription analysis. Analyses up to 10 times a day showed extraordinarily dynamic changes in gene expression during wort fermentation. Expression of some important genes was too short-lived to be defined by less frequent analyses. The lag between change in gene expression and its physiological manifestation suggests TRAC may be useful to monitor and control brewery operations.