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Location determination and high sensitivity gene detection using in situ branched DNA hybridization
Location determination and high sensitivity gene detection using in situ branched DNA hybridization
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机译:使用原位分支DNA杂交的位置确定和高灵敏度基因检测
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摘要
Methods are provided for highly sensitive and rapid in situ detection of a nucleic acid analyte of a known sequence. The method employs oligonucleotide probes in a series of optimized steps to amplify a signal and decrease background. Sensitivity is enhanced such that the method can detect as few as 1-2 copies of nucleic acid analyte per sample, the sample containing a cell, tissue or similar biological material. Methods of detecting and identifying the position of the nucleic acid analyte in a cell are also provided.
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