Procedure for the detection and localization of in situ hybridization using genes branched DNA.

摘要

A process for the in situ of a nucleic acid analyte within a sample of biological material comprising performing bDNA hybridization comprising the steps of detecting: (a) preparing a sample of biological material: (i) immobilizing the biological material on a substrate; (Ii) permeabilizing the substrate-bound biological material by contacting the substrate-bound biological material to a solution containing Proteinase K at a concentration of 0, 5 mig / ml to 50 mig / ml; (Iii) treating the sample with RNase so as to remove any RNA in the sample; and (iv) heating the permeabilized biological material to a temperature and for a time period effective to denature any dsDNA time.